Wed, 08 Dec 2021

New research on reading the mind of worms

22 Nov 2021, 19:55 GMT+10

San Diego [US], November 22 (ANI): Now, scientists can have a look at the brain activity of a microscopic worm and tell you about which chemical it smelled a few seconds ago.

This research was led by Salk Associate Professor Sreekanth Chalasani at the Salk Institute. This is a new study that can aid scientists in understanding the functions of the brain and integrating information.

The study was published in the 'PLOS Computational Biology Journal'.

"We found some unexpected things when we started looking at the effect of these sensory stimuli on individual cells and connections within the worms' brains," said Chalasani, member of the Molecular Neurobiology Laboratory and senior author of the new work.

Chalasani is interested in how, at a cellular level, the brain processes information from the outside world. Researchers can't simultaneously track the activity of each of the 86 billion brain cells in a living human -- but they can do this in the microscopic worm Caenorhabditis elegans, which has only 302 neurons.

Chalasani explained that in a simple animal like C. elegans, researchers can monitor individual neurons as the animal is carrying out actions. That level of resolution is not currently possible in humans or even mice.

Chalasani's team set out to study how C. elegans neurons react to smelling each of five different chemicals: benzaldehyde, diacetyl, isoamyl alcohol, 2-nonanone, and sodium chloride. Previous studies have shown that C. elegans can differentiate these chemicals, which, to humans, smelled roughly like almond, buttered popcorn, banana, cheese, and salt. And while researchers knew the identities of the small handful of sensory neurons that directly sensed these stimuli, Chalasani's group was more interested in how the rest of the brain reacted.

The researchers engineered C. elegans so that each of their 302 neurons contained a fluorescent sensor that would light up when the neuron was active. Then, they watched under a microscope as they exposed 48 different worms to repeated bursts of the five chemicals. On average, 50 or 60 neurons are activated in response to each chemical.

By looking at basic properties of the datasets -- such as how many cells were active at each time point -- Chalasani and his colleagues couldn't immediately differentiate between the different chemicals. So, they turned to a mathematical approach called graph theory, which analyzed the collective interactions between pairs of cells: When one cell is activated, how does the activity of other cells changed in response?This approach revealed that whenever C. elegans was exposed to sodium chloride (salt), there was first a burst of activity in one set of neurons -- likely the sensory neurons -- but then about 30 seconds later, triplets of other neurons began to strongly coordinate their activities. These same distinct triplets weren't seen after the other stimuli, letting the researchers accurately identify -- based only on the brain patterns -- when a worm had been exposed to salt.

"C. elegans seems to have attached a high value to sensing salt, using a completely different circuit configuration in the brain to respond," said Chalasani.

"This might be because salt often represents bacteria, which is food for the worm," he added.

The researchers next used a machine-learning algorithm to pinpoint other, more subtle, differences in how the brain responded to each of the five chemicals. The algorithm was able to learn to differentiate the neural response to salt and benzaldehyde but often confused the other three chemicals.

"Whatever analysis we've done, it's a start but we're still only getting a partial answer as to how the brain discriminates these things," said Chalasani.

Still, he pointed out that the way the team approached the study -- looked at the brain's network-wide response to a stimulus and applied graph theory, rather than just focusing on a small set of sensory neurons and whether they're activated -- paved the way toward more complex and holistic studies of how brains reacted to stimuli.

The researchers' ultimate goal, of course, isn't to read the minds of tiny worms, but to gain a deeper understanding of how humans encode information in the brain and what happened when this goes awry in sensory processing disorders and related conditions like anxiety, attention deficit hyperactivity disorders (ADHD), autism spectrum disorders and others.

The other authors of the new study were Saket Navlakha of Cold Spring Harbor Laboratory and Javier How of UC San Diego. The work was supported by grants from the Pew Charitable Trusts, the National Institutes of Health and the National Science Foundation. (ANI)

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